Journal: Cell reports

Article Title: Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

doi: 10.1016/j.celrep.2021.110202

Figure Lengend Snippet: (A) Phenotypic impact of Zn, PBT2, and PBT2 + Zn on S. pneumoniae 23F growth in cation-adjusted Mueller-Hinton broth (CA-MHB) supplemented as indicated. The data correspond with the mean (± standard error of the mean) absorbance 600 nm measurements from three independent biological experiments. Error bars, where not visible, are overlapped by the representative symbols. (B) RNA sequencing of S. pneumoniae 23F to determine relative gene expression, expressed as log 2 -fold change. Each dot represents a gene, distributed on the x axis in accordance with locus tag numbering for 23F. Genes more highly expressed in the presence of 8 μM PBT2 + 32 μM ZnSO 4 are present above the x axis (pink), with those below the x axis expressed at a lower level (purple). Genes of interest are annotated with their putative or characterized functions. (C and D) Whole cell metal ion accumulation of Mn (C) and Zn (D) in S. pneumoniae 23F in the presence of PBT2, Zn, and PBT2 + Zn grown in CA-MHB. Error bars indicate standard deviation of the mean from three biological replicates, ns = p > 0.05, ** = p < 0.01, **** = p < 0.0001, one-way analysis of variance with the Tukey post-test. (E) Development of resistance assays for S. pneumoniae 23F during serial passage in the presence of sub-inhibitory concentrations of PBT2 + ampicillin or vancomycin, as a positive control, in CA-MHB. Experiment was terminated at 15 days owing to a loss of viability in the presence of PBT2 + ampicillin. Data represent three biological replicates. (F) Enumeration of colony-forming units (CFUs) of S. pneumoniae from the lungs of BALB/c mice, following intranasal challenge with 5 × 10 5 CFU of strain DAW30 (n = 10). Colonization was examined at 24 h after the challenge. BALB/c mice were treated with combinations of ampicillin, PBT2, ampicillin + PBT2 or vehicle at 0 h and 6 h after infection via oral gavage (PBT2, vehicle) and/or subcutaneous ampicillin. Data represent the mean (± standard deviation) of two independent experiments with statistical analyses performed by the Mann-Whitney U test. ns = p > 0.05, *** = p = 0.001.

Article Snippet: S. pneumoniae Spain23F; ATCC 700669 multi-drug resistant Spain 23F ST81 lineage , ( ) , N/A.

Techniques: RNA Sequencing, Gene Expression, Standard Deviation, Positive Control, Infection, MANN-WHITNEY

Journal: Cell reports

Article Title: Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

doi: 10.1016/j.celrep.2021.110202

Figure Lengend Snippet: Combination of PBT2 and Zn resensitizes pathogenic S. pneumoniae strains to antibiotics of various classes

Article Snippet: S. pneumoniae Spain23F; ATCC 700669 multi-drug resistant Spain 23F ST81 lineage , ( ) , N/A.

Techniques:

Journal: Cell reports

Article Title: Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

doi: 10.1016/j.celrep.2021.110202

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: S. pneumoniae Spain23F; ATCC 700669 multi-drug resistant Spain 23F ST81 lineage , ( ) , N/A.

Techniques: Virus, Recombinant, SYBR Green Assay, RNA Sequencing, Expressing, Construct, Plasmid Preparation, Software, Microscopy, Targeted Proteomics

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Primer Sequences Used for Detection Extended-Spectrum Beta-Lactamase and Carbapenemase Genes

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Sequencing, Amplification

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Magnitude and Distribution of Carbapenemase Producing Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Comparison of Antimicrobial Resistance Patterns Between ESBLand Carbapenemase Producing and Non-Producing Enterobacteriaceae Isolates at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Comparison

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Gel image of the ESBL and carbapenemase genes from Enterobacteriaceae isolates at TASH 2019. ( A ) bla CTX-M (754bp), bla SHV (403bp) and bla TEM (293bp).

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Distribution of ESBL and Carbapenemase Genes Among Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: PLoS ONE

Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis

doi: 10.1371/journal.pone.0212045

Figure Lengend Snippet: Axiom Microbiome Array testing results of ATCC Metagenomic Control Material (ATCC MSA-4000).

Article Snippet: Neisseria meningitidis strain FAM18 (ATCC 700532) , 28.90 , 117,950 , Yes , Neisseria meningitidis FAM18 chromosome.

Techniques: Control, Plasmid Preparation

Journal:

Article Title: Production of Protective Human Antipneumococcal Antibodies by Transgenic Mice with Human Immunoglobulin Loci

doi:

Figure Lengend Snippet: Survival of CBA/N mice after infection with serotype 3 S. pneumoniae. (A) Survival of mice treated with MAb doses of 10 μg; (B) survival of mice treated with mAb doses of 1 μg. The y axes show percentages of mice surviving the number of days after infection depicted on the x axes. Mice were infected as described in the text after the administration of IgM (depicted by ×), PBS (#), MAb 1.2 (■), or mAb 1.10 (○). The experiments were performed twice, and the results of one experiment are shown. In panel A, IgM (x) and PBS (#) had identical survival.

Article Snippet: Mouse inoculations were performed as follows: groups of 10 mice each received 0.5 ml (1 or 10 μg in sterile saline) of MAb 1.2, MAb 1.10, the human myeloma IgM control (Calbiochem) used as an isotype control as described elsewhere ( 46 ), or PBS intraperitoneally; 1 h later, 0.2 ml (300 CFU in tryptic soy broth) of S. pneumoniae (ATCC strain 10813) was given via intravenous inoculation into the lateral tail vein.

Techniques: Infection